https://www.ema.europa.eu/en/documents/product-information/comirnaty-epar-product-information_en.pdf
Mechanism of action
The nucleoside-modified messenger RNA in Comirnaty is formulated in lipid nanoparticles, which
enable delivery of the non replicating RNA into host cells to direct transient expression of the
SARS-CoV-2 S antigen. The mRNA codes for membrane-anchored, full-length S with two point
mutations within the central helix. Mutation of these two amino acids to proline locks S in an
antigenically preferred prefusion conformation. The vaccine elicits both neutralizing antibody and
cellular immune responses to the spike (S) antigen, which may contribute to protection against
COVID-19.
https://en.wikipedia.org/wiki/Transient_expression
Transient expression, more frequently referred to "transient gene expression", is the temporary expression of genes that are expressed for a short time after a nucleic acid, most frequently plasmid DNA encoding an expression cassette, has been introduced into eukaryotic cells. The majority of transient gene expressions are done with cultivated animal cells. The technique is also used in plant cells; however, the transfer of nucleic acids into these cells requires different methods than those with animal cells. In both plants and animals, transient expression should result in a time-limited use of transferred nucleic acids, since any long-term expression would be called "stable expression".
Methodology varies depending on the organism to transform. While plants can be transformed with a construct introduced into Agrobacterium tumefaciens via agroinfiltration or floral dip, most animal cells would require a viral vector. In human, the field of transient transformation advanced rapidly during the 2020-2021 covid-19 pandemic with major COVID-19_vaccines using either direct mRNA transfer into human or adenovirus vectors, with the RNA being expressed in the host human to produce spike proteins that induce an immune response.
Some fungi, like yeasts or zygomycota, are susceptible to transformation by dedifferentiating their hyphae to protoplasts and adding Cl2Ca 10–50 mM, Tris-HCl 10 mM, polyethylene glycol (40%) and the DNA construct (> 5µg/ml) to a 10^8 protoplast/ml solution. Under this pH condition, PEG acts as a binder, promoting protoplasts to clump together and trap introduced DNA strands. This process has a relatively high efficiency, despite the fact that eukaryotic cells have abundant exonucleases that degrade exogenous DNA.
Voila ce que fait le vaccin Pfizer :
Tout d'abord que fait un antigène ?
Un antigène est une macromolécule naturelle ou synthétique qui, reconnue par des anticorps ou des cellules du système immunitaire d’un organisme, est capable de déclencher chez celui-ci une réponse immunitaire. Les antigènes sont généralement des protéines, des polysaccharides et leurs dérivés lipidiques.
Le vaccin fait produire des anticorps qui cible la protéine spike, protéine nécessaire au covid pour se propager dans les cellules de notre corps.
L'hypothèse que je me pose est la suivante : que se passe-t-il si la protéine spike produite par ce vaccin se loge dans les cellules de nos organes ? Puisque le vaccin nous fait produire des anticorps contre cette protéine Spike, la protéine étant désormais dans nos organes, est-ce que les anticorps attaque les cellules saines sans faire de distinction ?
